Physical and functional targets of RB and its paralogs p107/p130 have been studied largely in cultured cells, but the full biological context of this family of proteins' activities will likely be revealed only in whole organismal studies.
Our analysis of a large number of cultured melanoma cells identified largely uniform expression of XIAP and cIAP1, but not of cIAP2.
For instance, it has been noted that in vitro activated, cultured HSCs largely differ in their gene array profiles from in vivo activated HSCs [ 117].
Even after 6 h of treatment, β-catenin protein levels were largely unchanged in cells cultured in the presence of G-17.
Expression of TIMP-1 is up-regulated at the transcription level by various growth factors such as TGFβ1, whereas TIMP-2 is largely expressed constitutively by cultured cells [ 14].
Whereas characterization of integration patterns has largely been conducted in cultured cells, often with transposons delivered from transfected plasmids, transposition happens in vivo from resident transposon concatemers in mutagenesis screens.
To date, most studies exploring HAD pathogenesis [ 2- 6] have largely been confined to cultured cells, cerebrospinal fluid (CSF) and animal models, which can't mimic and reveal the breadth of in vivo cellular responses subverted and manipulated as a consequence of HIV infection.
Most mutations are completely destructive to FALDH catalytic activity [ 28], and the variation in residual enzyme activity measured in cultured fibroblasts largely reflects the presence of other aldehyde dehydrogenase enzymes that have some ability to oxidize long-chain fatty aldehydes [ 26].
We have noted above that it can be determined by sequencing that many of the non-cultured environmental organisms largely differ from those in standard culture collections.
However, these works are mostly based on cultured cell models, and the precise roles of EMT in lung cancer metastasis are still largely unclear.
What's the true story?' " There is no mention of Cohen's songwriting, though the narrator observes that Cohen is "not self-consciously cultured" and "listens largely to pop music".
