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Large-scale preparations of recombinant Ad-GR-eYFP and Ad-eYFP were prepared and purified using the Adeno-X virus purification kit (Clontech) and the viral titer was determined by plaque forming assays.
Large-scale preparations of plasmid DNA's HIVBr18 and the empty vector pVAX1 were prepared with the Endofree® Giga Plasmid Purification Kit from Qiagen according to manufacturer's instructions.
It is worth mentioning that the growth conditions are simple and also can be easily adopted for large-scale preparations.
The product crystallization followed by isomer epimerization strategy makes the reaction practical and useful for large-scale preparations.
Normal skin fibroblast cultures CRL-21277, ATCC) were used for large-scale preparations of normal procollagen.
The purified recombinant virus isolates were expanded for large-scale preparations in CEC.
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Based on these data, a purified large-scale preparation of AAV2/8BP2 was prepared and in vitro and in vivo transduction was assayed.
Of more lasting importance, though, were the discovery and large-scale preparation of sodium borohydride and lithium aluminum hydride.
In the early 1950s Spedding and his associates at the Ames (Iowa) Laboratory of the U.S. Atomic Energy Commission developed a practicable and efficient method for large-scale preparation of thorium metal.
The solution to the problem of large-scale preparation of elemental fluorine, which required the development and introduction of novel, fluorine-resistant materials of construction, made this important element generally available.
The method is simple and suitable for the large-scale preparation of high-quality CdS nanorods.
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