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Genome-wide or large-scale methodologies employed in functional genomics such as DNA sequencing, transcription profiling, proteomics, and metabolite profiling have become important tools in many metabolic engineering strategies.
To tackle this challenge, several large-scale methodologies, under the term functional genomics, have been developed which aim at revealing putative gene functions [ 1- 3].
There is a belief that the genomic revolution triggered by full sequencing of the human genome and technological development of large-scale methodologies announces the future of personalized medicine.
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In order to compare a 96-well plate mini-tube SSF approach with larger scale methodologies, comparative experiments were carried out using standard shake flask methods [ 12- 15].
He has an expertise in large-scale assessment methodologies, particularly in the area of complex sampling.
Finally, large-scale screening methodologies are being employed to detect other potential biomarkers that might be used to identify the rare ADPKD patient susceptible to tolvaptan-mediated liver injury.
But if corporations make it difficult to do repairs ourselves (e.g., glue down the battery), we are at their mercy, and must rely on large-scale production methodology.
We show that the integration of DNA-based quantification and large-scale sequencing methodology presented here produces unbiased data and allows the full determination of the whole pool of ligand sequences available after "n" rounds of selection.
This information, based on large-scale survey methodology, will provide critical insight into the potential broad and significant impact of obesity among the European population.
Indeed, initiatives such as the Cancer Genome Atlas (http://cancergenome.nih.gov) use large-scale profiling methodology to uncover cancer subtypes and further subtype specific treatment development (8).
We discuss several leading large-scale CO2 mineralization methodologies from a technical and engineering-science perspective.
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