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In addition, the hybridizing RNAs shown in Figure 5 are large transcripts of size similar to those recovered by RT-PCR suggesting that most likely there are no internal transcription initiation sites within the Tgm-Express1 element to generate truncated smaller transcripts.
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It produced a large transcript of the expected size (2,174 nucleotides) and exon structure (V1-ex19-27-V2).
The final construct MGBR2_ex19 27 produced a large transcript of 2,174 nt of the appropriate size and exon composition (Fig. 1) that was completely sequenced and consisted of V1, BRCA2-19-20-21-22-23-24-25-26-27 anomalyexons (canomalyl tranomalyt), without anomalymanomaly
The two largest transcripts of VPS13B in humans utilise alternate splicing to include either exon 28 (NM_017890) or 28b (NM_152564) which are homologous to dog exons 27 and 27b, respectively.
All four COLIGOs generated the large transcripts characteristic of RCT.
In the affected calf, there was, however, also an additional larger transcript of 5.8 kb present in all investigated tissues (Figure 3A).
Presumably, when there was no termination of transcription from the fadD2 promoter, fadD2 and fadD1 were transcribed together on the larger transcript of the same size observed on both blots (Fig. 3A and 3B).
This clone represents the largest transcript of PSG9.
Differential transcript expression of GIMAP8 was also observed with the four resistant animals predominantly expressing an approximately 3,300 bp transcript, although a larger transcript of approximately 4,100 bp could still be detected.
Availability of a large transcript database of the target species also highly favored the identification and characterization of targets.
Another reason could be the possible bias by overestimation of large transcripts and differences in the range of detection.
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