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The conjecture that the large inward-facing cavity observed in the apo structures is required to accommodate the large substrates of exporters has also been challenged (George and Jones, 2012) by the observation of smaller openings in the outward-facing conformations of MsbA and Sav1866 (Dawson and Locher, 2006, 2007; Ward et al., 2007).
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electrophoretic display based on a large substrate of 370 × 470 mm2 for the backplane process.
This region is only found in catB within its family, and it controls the access of large substrates to the active site [ 12].
With the increasing demand for nano/micropatterns on large substrates, the establishment of large-scale nanofabrication technology has become a priority.
However, in the presence of large substrates (full-length proteins) the substrate concentration has an effect in the oligomeric state adopted by DegP.
Here, gating elements control the access of large substrates by merging and dividing the space shared by the tunnels.
Therefore, the large substrate specifities of PoAbf, or its dual catalytic activity could illustrate the subfamily it belongs to.
However, one might expect that such large gates could be present in enzymes that catalyze reactions of very large substrates.
With the optimized combination of deposition parameters, homogeneous SiC coatings were deposited on relatively large area substrates of Φ50 mm and 50×50 mm with a deposition rate as high as 20 μm/min.
The task faced by the Tat system is mechanistically challenging because it involves transporting large protein substrates of differing size and surface properties across a membrane while maintaining the membrane permeability barrier.
The difficulty arises because, upon binding, the substrate wraps around the circumference of BoNTAe constituting an unusually large substrate enzyme interface area of 4,840 Å2 (Figure 1) [17].
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