In Texas, in other words, you are prohibited from walking down the street with a small revolver in a holster on your hip but entitled to be out in public with a large shotgun slung over your shoulder.
Our strategy was to employ a simpler version of the approach used by TIGR to assemble large shotgun sequencing projects [20].
Due to the enormous quantity of short DNA fragments in large shotgun sequencing-derived or pyrosequencing-derived metagenomic datasets, methods have been developed that are more suitable for taxonomic binning than the analysis of highly conserved phylogenetic marker genes.
In summary, our results suggest that the design of large shotgun proteomics studies should focus on efficiency not only to save resources but, most importantly, to yield reliable peptide discoveries.
A total of 2 μg of ligated metagenomic DNA was electro-transformed into the E. coli TG1 electrocompetent cells to obtain the pilot shotgun library, while the rest of the ligation mixture was used in 27 separate transformation reactions to generate a large shotgun library and overcome a problem of promiscuous (fast growing) clones.
I heard a gun being cocked, and suddenly all eight cops poured around the corner, racing out after this guy the last of whom was a giant, seven-foot-tall black guy carrying the largest shotgun I've ever seen.
Large scale shotgun sequencing approaches allow the discovery of many novel genes found in the environments independent of cultivation efforts [5] [8].
One of the flaws of large-scale shotgun proteomic studies is that dominant peptides can skew the results in a manner that causes some proteins to remain undetected.
To date, in vivo proteomics of the tubercle bacillus has not been described and this work has generated the first large-scale shotgun proteomic data set, comprising over 500 unique protein identifications.
We present a method called MSpresso that improves our ability to identify proteins in large-scale shotgun proteomics experiments.
