For this analysis, qRT-PCR was performed on 20 selected genes instead of large scale profiling.
Large scale profiling experiments have indicated that most miRNA SNPs occur outside of the pre-miRNA region.
Microarray technology provides a comprehensive, rapid and efficient method for large scale profiling of gene expression changes in biological samples (e.g., treatment versus control, disease versus normal).
To our knowledge, this kind of large scale profiling of immune function has not been done previously on greenfinches or any other passerines.
Large scale profiling revealed a global alteration of miRNA expression patterns in human cancers (Lu et al, 2005; Murakami et al, 2006; Kida and Han, 2008).
The success of large scale profiling approaches is exemplified by the first detection of IDH1 mutations in a study that sequenced 20,661 genes in 22 human glioblastoma samples [ 95].
In a recent study by Dudziec E. et al. [ 24], a large scale profiling among DNA methylation, histone modification and gene expression using UC cells revealed that 20-30% genes were silenced by epigenetic regulation.
Only one paper, which focused mainly on the fungal partner Laccaria bicolor, has documented large scale profiling of the molecular events during ECM establishment with a gymnosperm as plant host [ 26].
This large scale profiling technique applied to patients with schizophrenia has already shown a surprising number of genes related to energy metabolism, oligodendrocyte function and myelin formation that are associated with psychopathology [ 261].
With application of the pultrusion method, the manufacture of large-scale profiles with various cross-section forms became potentially possible with relatively low costs.
In vivo methods include the use of mass spectrometry to generate large-scale profiles of cellular phosphoproteins (reviewed in [10], [11]).
