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This treatment is strongly recommended for medium- and large scale preparations and is optional for syntheses on small scale where the concentration of acrylonitrile generated in the deprotection mixture is low.
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These new supports combine easy monitoring, high loading capacities, large scale preparation, and homogeneous kinetics characteristics while keeping advantages of solid-phase synthesis including easy purification and workup.
Using streamlined and centralized manufacturing of CIK cells, including large-scale preparations and cryopreservation, these cells could probably be commercialized anywhere, thereby increasing convenience and flexibility for cancer patients.
Large scale preparations of each C. trachomatis isolate were prepared and EBs were purified as described [ 37].
Plaque isolates were purified three times and expanded for large scale preparations in Vero cells.
Once expression and purification was established we checked whether the large scale preparations represent also catalytically active enzyme.
Large scale preparations used the same conditions.
The described method allows the use of large scale preparations providing greater probability of detecting genes present in low abundance in the soil environment.
The merits of the methods in enhancing enzyme stability at adverse conditions and their potential for large-scale preparation and the use of the nanostructured biocatalysts are discussed.
The as-revealed simple design, easiness to large-scale preparation and superior electrocatalytic performance enable the bifunctional Ni/Co-S act as promising electrode materials for overall water splitting.
The purification method and identification results can provide basis for large-scale preparation and widely application of lipopeptides.
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