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DNA fragments containing the 148 bp 601 high-affinity nucleosome positioning sequence and an additional length of flanking DNA were amplified using large scale polymerase chain reaction followed by purification of the amplified fragment.
To solve these issues, we developed a sensitive, specific and rapid gene chips for screening prevalent point mutations of HBV on a large scale, polymerase chain reaction (PCR) for detection of genotypes and mutations in pre-core and basic-core promoter.
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Screening for the presence of NPHV in other mammals, the authors performed large-scale polymerase chain reaction (PCR -based investigation of samPCR -baseddogs, cats, pinvestigation dofkeysamplesorses.
Transcription reactions (50 μL) were performed with 1 μg of purified PCR products and T7 RNA polymerase enzyme, in T7 RiboMAX™ Express large scale RNA production system kit (Promega) according to manufacturer's protocol.
Traditional methods to detect RNA processing, such as Northern blotting, reverse‐transcribed polymerase chain reaction and primer extension assays, are difficult to apply on a large scale.
Polymerase chain reaction may increase case ascertainment, but basic microbiologic testing on a large scale is required.
Probes were made by use of T7 RNA polymerase; substrates were PCR products obtained using T7 and SP6 primers applied to cDNA clones derived from a large scale Xenopus tropicalis EST project [23].
"Alyse Rosner: Large Scale Work".
He does this on a large scale.
The people who were thinking large scale and small scale.
Large Scale Biology Corporation.
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