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S. cerevisiae protein abundance measures were averaged from four quantitative large-scale measurements: two large scale measurements in two conditions (Newman et al. 2006), and a large-scale protein abundance measurement from two sources (Ghaemmaghami et al. 2003; Lee et al. 2011).
Upon control of several experimental conditions, careful and stringent spectral assignments, and sophisticated normalization procedures, it appears that MS-based quantification can provide an attractive and sensitive tool to obtain large scale measurements of relative protein concentrations.
mRNA levels were determined by averaging large scale measurements of mRNA levels (Wang et al. 2002; Ingolia et al. 2009).
A goal of systems biology is to reconstruct these underlying networks and to infer associated biological phenomena from large scale measurements [ 3].
The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measurements.
Currently, there are less than a few dozen large scale measurements of protein levels, while there are >25,000 sequenced genomes.
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His research interests include computer security and network measurement, with an emphasis on both large scale attacks and large scale measurement.
However, large scale measurement of mRNA levels and specifically protein abundance (PA) are not available for most of the organisms with sequenced genomes.
However, there is a lack of large-scale measurements of physical activity patterns across free-living populations worldwide1,6.
The first large-scale measurements of the way humans play Rock-Paper-Scissors reveal a hidden pattern of play that opponents can exploit to gain a vital edge.
Globally, millions of deaths each year are associated with physical inactivity but our understanding of activity patterns in different populations remains limited owing to a lack of large-scale measurements.
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