Sentence examples for large scale expression in from inspiring English sources
One positive colony was selected to a large scale expression in a 3 litres BioFlo 110 laboratory fermenter (New Brunswick Scientific).
6-His-hCNTF construct was chosen for large scale expression in Rosetta 2 (DE3)pLysS with the auto-inducing media TBONEX. 2 X 450 ml of cultures were inoculated with overnight seed cultures (30 1) and grown at 25°C with shaking at 270 rpm for 30 h.
Large scale expression was performed in 500 ml in baffled 2 l Erlenmeyer flasks at 15°C for 16 h with a final IPTG concentration of 0.1 mM.
For large scale expression, scFv was cultivated in LB supplemented with 25 μg/ml of kanamycin in lab fermenter scale (1 5 l culture volume respectively).
An in-house initiative to develop large-scale expression profiling in Arabidopsis thaliana provided a large database from which to select candidate targets for absolute quantification.
So far, systematic large-scale expression profiling in CHO cells by microarray was limited due to lack of publicly available array designs and limitations of alternative approaches.
First, the datasets were normalized using quantile normalization to ensure that inherent large-scale expression differences in the datasets based on different sources and laboratories were minimized.
Initial attempts at large-scale expression profiling in FFPE tissues yielded either poor reproducibility and sensitivity [28] or loss of gene signature information when compared with matched FF samples [29].
Analysing large-scale expression measurements in the context of molecular pathways or interaction networks can reveal key regulatory molecules and functional modules involved in the disease process and suggest hypotheses regarding the system response to perturbation.
Large-scale expression profiling in leaves has identified TPs from many different classes that are up-regulated during the senescence of Arabidopsis (Arabidopsis thaliana) leaves (van der Graaff et al. 2006), but fewer TPs have been identified in petals.
We applied the method to analyze the large-scale expression data in Arabidopsis thaliana taken from TAIR (Swarbreck et al., 2008), where 1388 samples and 22 746 probes were available for the analyses.
