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With the more common large repeats, it is therefore difficult to design an antigenomic molecule that will bind selectively under physiological conditions.
Mapping studies have shown that Brassica hirta lacks large repeats as well [38].
This trend is particularly evident in rosids, in which coverage by large repeats does not exceed 6% for any one species, and in which the two largest sequenced mitochondrial genomes (Vitis and Cucurbita) contain no large repeats (Fig. 2).
Removed SNP detected with a coverage of more than 12 reads (to eliminate paralogs, large repeats and gene families).
In MIV, this was due primarily to the presence of fifteen large repeats representing about 20% of its genome.
Apart from large repeats like the rRNA operons, local inverted repeats like transposases also characterize genomic complexity and thus have an effect on the assembly process.
Coverage by large repeats varies considerably within grasses and underlies substantial changes in sequence complexity between relatively recently diverged taxa (e.g., Oryza and Bambusa) as well as subspecies (Fig. 2) and genetic lines [2] of maize.
By contrast, eudicot mitochondrial genomes show greater disparities in genome size, but with the exception of the male-sterile genetic line of Beta, lower overall coverage by large repeats (Fig. 2).
BlastN was used to discover large repeats.
Large repeats are mostly represented by IS (Insertion Sequences).
Apparently, these small repeats were part of the large repeats.
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