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Aluminum planchettes [21], microscope grids [18], cellulose capillary tubes [20], [22], sapphire discs [14], [23] and aclar discs [24] have been used with satisfactory results for a large panel of cell types.
We treated a large panel of cell lines (n = 36) with our Nek2 inhibitors.
This analysis has now been expanded to a large panel of cell lines (Table 1).
We performed a high throughput combination screen on a large panel of cell lines and searched for markers of sensitivity or resistance.
Nuclear secreted and transmembrane molecules regulate the proliferation and differentiation of a large panel of cell types during embryogenesis and adulthood and are also potentially involved in wound healing.
In this study, we used a large panel of cell lines to derive an EMT signature specific to each cancer type, and hence, it is less likely that the derived signature contains features unique to a single cell line.
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A faster and more reliable system able to screen large number of potential compounds in a large panel of cells would therefore be considered an enormous improvement in the pharmacology research.
Furthermore, recent results utilizing very large panels of cell lines indicate that they also to a large extent retain genomic features of the primary tumor and can recapitulate clinical findings with regard to their response to targeted inhibitors [ 9].
Our identification the Tsci transcripts supports efforts put forth by the ENCODE consortium and others to perform RNA-Seq across large panels of cell and tissues (Yue et al. 2014).
Further experiments that might include tests on a larger panel of cell types are warranted to establish or disprove this postulate.
Effective inhibitors identified were then further tested against a larger panel of cell lines in 3D, including non-transformed EP156T and RWPE-1 cells, and non-invasive DU145, LNCaP and 22rV1 cells (Figure S5).
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