The replication origins of the budding yeast S. cerevisiae, also identified as ARS (autonomously replicating sequences), are ∼200 bp (or longer) and contain an essential 11-bp ARS core-A consensus sequence (ACS) [30], [31], extended to a 17-bp motif when based on a larger number of origins [32], [33].
However, rather than losing origins, dormant origins were now activated so that a larger number of origins contributed to DNA replication.
Together, these observations indicate that while most mutants display a smoother profile characterized by a lower number of active origins, deletion of mrc1 sharpens the profile, with a larger number of origins significantly contributing to DNA replication.
The sequence-independent distribution of ORC-binding, pre-RC-assembly, and initiation patterns indicates that a large number of silent origins are present in the mini-EBV genome.
Due to the large number of possible origins and destinations within the country, we used a gravity model integrated over individual destinations that only retained information on the distances travelled.
This could only occur if a large number of replication origins initiated DNA replication following Cdc6-E224Q removal.
As mentioned above, based on x and y components, a large number of replication origins have been located in both bacterial [ 12, 13] and archaeal genomes [ 14].
The increased organizational complexity of the eukaryotic origin definition and MCM recruitment system is likely a consequence of the increased regulatory demands imposed by the large number of replication origins found in eukaryotic chromosomes (Diffley, 2011).
The detailed microscopic observations of tissue slices including the hippocampal formation of epileptic animals showed the presence of quite a large number of inclusions of unknown origin.
As PBs are delimited by ER membrane, this attractive force between chloroplasts and ER could be the reason for the identification of large number of proteins of chloroplastic origin in the PB proteome.
