This suggests that the large isoform is important for the maintenance of a subset of the stereocilia: in particular, it maintains the stereocilia are involved in converting sound energy into an electrical signal in the inner part of the cochlea.
Interestingly, in WT brain the Scn9a transcript had two isoforms, but in NOVA DKO brain the larger isoform was lost.
However, upon stimulation with VEGF we observed that only the larger isoform is translocated to the nucleus.
Interestingly, following SeV H4 but not SeV WT infection, the larger isoform was degraded, whereas the shorter isoform was not affected.
The larger isoform is also more abundant in brain and spinal cord (Claudiani et al., 2005), specifically in neurons (Charvin et al., 2003).
The large KASH isoforms are expected to localise at the ONM because of size restriction of protein transport across the NE, however the smaller variants are probably capable of embedding within either membrane.
In human breast tissue, the LAP isoforms are larger (approximately 42 46 kDa) and the LIP isoform remains unchanged at 20 kDa.
JNK1 α1, JNK1 β1, JNK2 α1 and JNK2 β1 isoforms all have a molecular weight of 46 kDa, whereas JNK1 α2, JNK1 β2, JNK2 α2 and JNK2 β2 isoforms are larger due to an extended C-terminus and their molecular weight is 54 kDa.
Probes designed to the individual sept9a transcripts were not synthesized because the unique regions of the longer sept9a isoforms are not large enough to probe individually.
A large proportion of aquaporin gene isoforms are predominantly expressed in roots and their activity can regulate the water flow across the root [ 32].
