Sentence examples for large insertion mutagenesis from inspiring English sources

However, in a large insertion mutagenesis screen, a zebrafish mutant harboring an insertion in the first intron of cofilin 1 (cfl1) (previously known as cofilin 2 like) was identified and showed no obvious phenotype at one day after fertilization [30].

With the development of a highly efficient, piggyBac transposon-based mutagenesis system for P. falciparum, a large-scale, genome-wide insertion mutagenesis approach could aid greatly in dissecting the P. falciparum genome [15], [16].

However, for large-genome and transformation-recalcitrant species like barley insertion mutagenesis strategies based on transposable elements are likely to be advantageous.

Thus, an ideal reporter gene needs to tolerate insertion mutagenesis of relatively long inserts and to not exhibit sequence discrimination.

Therefore, the AT method may affect more genes than classical insertion mutagenesis.

To retain the AaargB sequence in the arginine auxotroph, the AaargB function was disrupted by insertion mutagenesis.

The intracellular process for protein expression is much simpler and there is no potential risk of insertion mutagenesis.

However, the conventional directional coupler has relatively large insertion loss.

However, such a filter typically introduces large insertion losses.

Insertion mutagenesis of APEC strain IMT5155 was performed randomly by using transposon pUTmini-Tn5km2 as previously described [20].

After constructing a library of LacI-GFP insertion proteins (LacI-GFPi) using TAGIT, we identified six sites in LacI that are tolerant to GFP insertion, including those previously identified by epitope insertion mutagenesis.