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So, ECCN 3 represents the best tradeoff between having enough nanoparticles to precipitate on (or coat on) versus decrease in enzyme activity due to excess large immobilization surface.
Such bimodal mesoporous composites show a large immobilization capacity for the biomolecules of cytochrome c (up to 732 mg/g) and lysozyme (up to 790 mg/g).
Thus, a reduction of this coefficient, often under critical conditions such as joint large immobilization periods of time [ 38, 39] entails a decrease of fluid flow into the cartilage porous matrix and therefore implies smaller deformations.
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The purpose of this experiment was to determine possible faults in the splint design, thereby facilitating preparations for performing further studies involving more wearers, longer durations, and larger immobilization regions.
Clearly, the immobilization or trapping incidents were short-lived in comparison to functionally relevant binding events, as in the case of splicing factors, which displayed a threefold larger immobile fraction with average binding times of >60 ms (34).
On one hand, the biosensor could retain the bioactivity of Lac to a large extent by immobilization of Lac with silica spheres on the MWCNTs/SPE.
In our survey, we found a large preference for immobilization of the shoulder in internal rotation position.
However, if the observed hole is large or if the immobilization area overlaps with the edge, there still exists a chance that the covering surface would be distorted.
Compared with pure silver nanoparticles, Ag-SiO2 nanocomposites could provide a large room for the immobilization of HRP-anti-AFP, and improve the electrochemical responses of the immunosensor.
The mesopores of the nanomaterials spanned from 20 to 60 nm in diameter and post-functionalization allowed the electrostatic immobilization of large proteins (e.g. mTFP-Ferritin, ~ 534 kDa).
DNAzyme (as a kind of peroxidase mimic) could catalyze the oxidation of 4-CN, whilst AuPd hybrid nanostructures could not only provide a large surface coverage for immobilization of biomolecules but also promote 4-CN oxidation to some extent.
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