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qPCR revealing ubiquitous B4GAT1 and LARGE expression in all tissues analyzed, with highest expression of LARGE in brain and heart.
It had also been shown that ectopic LARGE expression in pgsI-208 CHO cells did not induce α-DG hyperglycosylation (Inamori et al., 2012; Ashikov et al., 2013), consistent with the fact that UDP-Xyl is essential for synthesis of the LARGE glycan in vivo.
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Either algorithm can be coupled with the incremental reformatting and screen update algorithm developed in this thesis to produce an interface capable of correctly updating and displaying changes to relatively large expressions in real-time.
However, of all the differentially expressed genes, ependymin was a clear candidate and stood out as having the largest difference in expression levels between hierarchical levels, with ∼2-fold higher levels in sub-dominant than dominant, a 6.6-fold difference between sub-dominant and subordinate, and a 5.5-fold larger expression in the subordinate compared to the dominant.
In the six libraries, miR165a showed the highest sequencing reads, which may suggest the largest expression in pear fruit among miRNA families.
In this data set, parent node mutations resulted in large expression changes in their direct targets.
The genes have large expression ratios in the OneRA that are reduced in the TwoRA.
Our results suggest that ratios are most commonly reduced among the genes with large expression ratios in the OneRA, thus likely to be differentially expressed.
Other technologies, including RNA sequencing, are also generating large expression datasets in multiple species.
The WEE1 gene harbours a large expression level in the pCR group.
We were aware of 13 large expression sets in human, mouse, and rat.
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