Pertea, G. et al. TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets.
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A modified approach was designed to cluster and assemble large EST datasets, aiming to detect alternative splicing together with EST abundance of each splicing variant.
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This is the first large EST dataset available for analyzing the Xiphophorus trancripts.
While a discernible homolog could neither be identified in the genome of the sea anemone Nematostella vectensis, nor in a large EST dataset from the symbiotic sea anemone Aiptasia pallida, we identified SCRiP sequences in multiple scleractinian coral species.
In details, this large EST dataset of Epimedium is a powerful resource for further studies.
Of the previously identified large EST clusters [ 27], 28 occurred in the L2 stages.
We have described the generation of a large EST dataset from regenerating newt hearts.
Web-based tools are most convenient for biologists to effectively process large EST data sets.
Both legume model plants have relatively large EST collections (over 150,000 sequences).
