Sentence examples for large depth of coverage from inspiring English sources

Given the large depth of coverage of the newly obtained genome sequence, and the successful verification of a subset of bases in conflict, we accounted for revisions to the HdrR reference sequence based on the short read data in the subsequent analysis of all other samples in this study.

The larger depth of coverage gives more reliable polymorphic data reducing the impact of low quality reads and the problems of mapping to regions of repetitive DNA.

However, the detection of larger structural variants based on depth-of-coverage (DOC) [ 19] or paired-end mapping (PEM) [ 20, 21] methods could not reduce the SV size and localization resolution to the one base pair level.

Nodes with a small nucleotide distance and relatively equal depths of coverage will be connected by an edge weighted with a very small distance in the graph, whereas nodes separated by a large nucleotide distance and/or a large difference in depth of coverage will be connected by an edge weighted by a large distance.

The largest SAR11 rRNA-anchored scaffold reconstructed with the Celera Assembler was relatively small (ca. 21,000 bp), and assembly depth-of-coverage was low (0.94 – 2.2 fold) [ 7].

For a given genomic sequence, this method assesses depth-of-coverage and compares it with the average coverage depth.

Finally, with the goal of mapping as many different loci as possible, emphasis was given to breath of coverage (i.e. 1 SNP per gene for a large number of genes) versus depth of coverage (i.e. as many SNPs as possible per gene for a low number of genes).

The largest scaffolds showed a uniform depth of coverage in the shotgun sequencing project (Fig. 4 in [ 3]) and are thus clearly composed of MAC-destined DNA.

An additional 147 Mbp of large deletions were identified with a depth of coverage approach.

While mapping of single-end or concordant paired-end sequences can be useful for determining copy number variation (CNV) of genes using depth of coverage, the large copy number of most TE families excludes this approach, as the variations in copy number would be insignificant with respect to the total number of copies.

Given the large number of reads and high depth of coverage, digital normalization was applied to the trimmed reads prior to assembly with Trinity (Grabherr et al. 2011; Haas et al. 2013) and Velvet Oases (Zerbino and Birney 2008; Schulz et al. 2012).