Microarray data repositories as well as large clinical applications of gene expression allow to analyse several hundreds of microarrays at one time.
Here we present the first large scale clinical application of our proteomics technology to discover blood protein biomarkers in a large multi-center case-control study conducted in archived samples from 1,326 subjects from four independent studies of non-small cell lung cancer (NSCLC) in long-term tobacco-exposed populations.
The RBP4 MSIA demonstrated in this study is both high throughput and automated (ideal for large scale clinical application), and since the MS analyses are performed on the intact immuno-affinity captured protein there is no need for time consuming and costly proteolytic digestion.
Although it performs well in large cohorts, clinical application is limited owing to the poor specificity of the measurement.
Future research is needed to investigate whether an equally marked improvement can be achieved in a large scale clinical application study, ideally one comprising all the departments involved in admission and assignment planning.
Cryopreservation of tissue engineered bone (TEB), whilst maintaining its osteogenic ability, is imperative for large-scale clinical application.
It is believed that when only a new non-viral method is explored, the large-scale clinical application of gene therapy would be allowed.
Despite the tremendous improvement in efficiency and efficacy of biomaterials-assisted regenerative medicine, underlying therapeutic mechanisms have not been clearly understood, which renders safety a main concern for large-scale clinical application.
Here we present a comprehensive clinical biomarker study of lung cancer and the first large-scale clinical application of a new aptamer-based proteomic technology to discover blood protein biomarkers in disease.
Transplantation of cardiac stem/progenitor cells to improve cardiac function is theoretically plausible, but limited cell sources and possible rejection in allogeneic transplantation prevent large-scale clinical application.
