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Flow cytometry is a powerful tool for quantitative biology because it can perform single-cell analysis of large cell populations using multiple parameters.
Stem cell-derived populations may allow the creation of large cell populations that have increased replicative potential over adult differentiated cells.
These measurements, performed over large cell populations, show quite generally that sequence-specific transcription regulators with well-defined protein-DNA consensus motifs bind only a fraction among all consensus motifs present in the genome.
After dynamic seeding followed by 5 days of static culture gyroid scaffolds showed large cell populations in the centre of the scaffold, while salt-leached scaffolds were covered with a cell sheet on the outside and no cells were found in the scaffold centre.
The models are designed for large cell populations.
Many methods used in cell biology are based on bulk measurements on large cell populations.
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Studies of cell contraction within three-dimensional constructs typically calculate an average contractile force from the gross deformation of a macroscopic substrate by a large cell population.
The uniform-gradient magnetic field applies nearly uniform forces to a large cell population, permitting statistical quantification of select molecular responses to mechanical stresses.
Each dot on the plot is a single cell from the culture; each flow cytometer plot is therefore a comparison of a large cell population.
To assess relative RNA localizations we developed a software dedicated to distance measurement between two fluorescent probes and allowing analysis of a large cell population.
High-throughput flow cytometry probing immunolabeled phosphoproteins [6], [7] allows multiparameter sampling of protein activation state across a large cell population, but requires serial analysis of samples, hence performing sequential assays of every experimental condition or timepoint – a key limitation when performing genome-scale screens.
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