As the conclusion, decane induction by continuous feeding would be essential for large scale biotransformation of methyl decanoate to sebacic acid.
These studies are particularly valuable to industry due to possibility of carrying out large scale biotransformations.
These successful case stories demonstrate the unexploited potential of oxidoreductases in medium and large-scale biotransformations.
Even though the whole cell-catalyzed reduction of o-chloroacetophenone presents an extreme case of catalyst instability, the problem of catalyst inactivation by hydrophobic substrates is common to a large number of biotransformations in organic synthesis (Burton et al., 2002; Doig et al., 2002; Kizaki et al., 2001; Kratzer et al., 2011; Pfruender et al., 2006; Schmid et al., 2001).
Pseudomonas putida KT2440, a microbial cell factory of reference for industrial whole-cell biocatalysis, is unable to support biochemical reactions that occur under anoxic conditions, limiting its utility for a large number of relevant biotransformations.
Although there are several reports showing the employment of aqueous two-phase systems (ATPS) for biotransformation in large-scale, the industrial application of ATPS is still hampered by their drawbacks such as slow diffusive mass transfer, long settling time for phase separation, and batch processes.
The challenge is to develop system suitable for carrying out biotransformation of larger, more polar, and multifunctional compounds.
CYP2D6 is involved in the biotransformation of a large number of drugs, including risperidone.
The largest fold induction of a biotransformation transcript was observed in the naïve N. bryanti, which induced sulfotransferase 3A1 by 22×.
This biotransformation is achieved in large part by cytochrome CYP3A.
