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Gestwicki chemically tethered a synthetic ligand for FKBP--a large binding protein that soil bacteria use to cripple enzymes in bacterial foes--to Congo red, a dye that sticks to β amyloid.
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The changes in the Faradaic impedance of gold/biomolecules system due to specific binding of small molecule to a significantly larger binding protein molecule were investigated.
The Solyc04g010040 gene codes for an RNA recognition motif-containing protein and belongs to the large RNA binding protein family whose members are known to be involved in regulatory processes in response to different abiotic stress conditions (Ambrosone et al. 2012).
A maximum of 120 matching spheres were used to ensure adequate ligand sampling within large binding surfaces like protein-protein interfaces.
Axonal regions and muscle cells contain high concentrations of cytoskeletal components that may restrict or slow considerably the movement of larger calcium binding proteins compared to large snail neurones.
Optimization of a serotype-selective, small-molecule inhibitor of botulinum neurotoxin serotype A (BoNTA) endopeptidase is a formidable challenge because the enzyme-substrate interface is unusually large and the endopeptidase itself is a large, zinc-binding protein with a complex fold that is difficult to simulate computationally.
Another part of the challenge in developing and optimizing these inhibitors is the endopeptidase itself, which is a large zinc-binding protein with a complex fold that is capable of large-scale conformational changes in solution and that is therefore difficult to simulate computationally [17].
We present a method of labeling and immobilizing a low-molecular-weight protein, calmodulin (CaM), by fusion to a larger protein, maltose binding protein (MBP), for single-molecule fluorescence experiments.
On the quantitative level the agreement between the two methods in the case of majority of proteins is good, only for 2 proteins the ratio differences are larger (for Retinol binding protein (RBP) ratio 4.6 for MRM and 2.65 for iTRAQ).
ROOT HAIR DEFECTIVE 3 (RHD3), a large GTP-binding protein, is ubiquitously expressed in plants.
Ribulose-1, 5-biophosphate carboxylase activase and Rubisco large subunit-binding protein have been shown to play a critical role in the activation of Rubisco [ 33].
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