Sentence examples for large amplification products from inspiring English sources

Saturation reverse transcriptase (RT) PCR of each splicing boundary in brain and liver mRNA specifically detected normally spliced amplicons in wildtype (wt) and heterozygous (ht), but in homozygous tissues (hm) a smear of abnormally large amplification products was found for the exon 5 6 boundary suggesting the presence of intron 5 including the Neo selection marker (Fig. 1F).

A possible explanation for the lack of interclone variation is that relatively large amplification products could be retrieved reproducibly from other loci such as cytochrome b (377 bp) as well, demonstrating the relatively good preservation state of most of the ancient materials used in this study [ 12].

The optimal target regions were defined by the CODDLE program using the following criteria: a) mutations close to primers (~10% of target sequence) are not readily detected, particularly in large amplification products b) maximize exons and/or intron-exon splice junctions and c) maximize regions encoding for conserved domains within the protein.

These F1 mice were genotyped using PCR primers that flanked the targeted exon and the residual loxP sequence; the targeted allele, which included the single surviving intronic loxP sequence, gave a larger amplification product than the wild-type allele (Figure 1).

In one case, OMTai23658, the larger amplification product could not be obtained.

Transformation efficiency (number of transformants/µg of plasmid) for low, medium and large sized amplification products.

The fact that some of the material does not enter the gel might be explained by extremely large size of the resulting amplification products and/or by the association of the amplified DNA with proteins.

Since a large amount of amplification product is created by the LAMP reaction, precipitate of a size that can be easily confirmed with the eyes is generated when PEI is added to the LAMP reaction solution.

This detection method effectively utilizes the characteristics of the LAMP method, i.e., a large amount of amplification product can be synthesized in a short time while maintaining high specificity.

These large cDNAs are strongly underrepresented in control amplification products of unfractionated cDNA (figure 2, panel A).

Microarray platforms based on either oligonucleotides or purified amplification products have been utilised in parallel to produce large amounts of data.