In this respect, large amounts of template DNA were used and multiple amplifications examined, as recommended [ 17].
Sanger sequencing of BAC-ends restrictedstoicted to a 96 well format in many sequencing centers, because of low template yields and large amounts of template used for the sequencing reactions.
The conventional genotyping method used for this polymorphism (multiplex PCR with fragment detection by gel electrophoresis) is laborious and requires large amount of DNA template.
Restriction digestion adds an extra step and requires larger amounts of template DNA.
The potential for contamination or mix-ups between samples increases rapidly with the number of steps or genotyping reactions required, and the approach also requires a large amount of resources (time, reagents, template DNA).
This protocol is specific for foot-and-mouth disease virus (FMDV) or other polyadenylated RNA viruses and circumvents both the use of PCR and the requirement for large amounts of initial template.
1-D gold nanostructures [GNSs] were mainly obtained in the mesopores when a small amount of TiO2-containing mesoporous SiO2-TiO2 was used as a template, whereas the use of a template containing a large amount of TiO2 led to the formation of shorter 1-D or 0-D GNSs.
Moreover, a synthetic ligation template is required to recircularize and monomerize the tandem repeated complements, so this technique requires the additional production of large amounts of ligation templates.
Since the provision of thermally generated electrons from TiO2 increased when a template contained a large amount of TiO2, Au ions were rapidly reduced and deposited as shorter 1-D or 0-D GNSs.
The prepared carbon material has uniform pore structure as CMK-5 type and retained a relatively large amount of nitrogen species after pyrolysis and removal of the silica template.
