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The single gene global alignments were concatenated to generate a single large alignment for the 9 organisms.
The different multiple alignments were concatenated into one large alignment for which a distance matrix was calculated based on Poisson correction using the software package TREECON [ 34].
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The best reconstructions, at 5.4 Å resolution, were obtained when using averages of six or four individual video frames, which apparently represent the optimum between accurately describing particle movement and not introducing too large alignment errors for these data.
The values of τ for each branch were calculated for several large alignments of amino acid sequences from various taxonomic groups (133 nuclear proteins from eukaryotes [ 37], 146 nuclear proteins from animals [ 38], 45 proteins from Archaea [ 39], 57 proteins from Bacteria [ 40], 13 mitochondrial proteins from deuterostomes [ 41] and 50 proteins from plastids and cyanobacteria [ 42]).
This information ensures that BAM search operations (which can be lengthy for large alignment files) can be avoided.
Because ML phylogenetic inference is NP complete [6], no practical method can guarantee that it will find the optimal topology for a large alignment.
– Given a polynomial time algorithm for finding a largest alignment of RMSD below a threshold d, one can efficiently compute an alignment of a given size ℓ which minimizes the RMSD (Theorem 7).
As shown by the previous analysis, PAGAN has the potential to scale up to large alignment extension tasks while its approach for modelling of insertions/deletions and uncertainty is especially well-suited for analyses of short and noisy NGS data.
The sequence alignments for all 12 proteins were concatenated into a single large alignment containing 8899 positions.
Here, we describe FastTree 2, a tool for inferring ML trees for large alignments.
This makes them fast for short alignments (small L) but slow for large alignments.
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