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Time lapse series with chemical contrast allow us to follow and interpret the chemical processes in detail.
We acquired a time lapse series of cortical slices in a growing cell (Figure 4).
Confocal time lapse series were then collected.
For time lapse series YFP and chlorophyll were both excited at 488 nm and pictures were collected without frame averages.
Time intervals were 11 hr for tomato and 24 hr for A. thaliana and C. rubella time lapse series.
To determine if Hsp17.6 expression oscillates we examined the time lapse series from Hsp17.6p GFP roots which had been heat shocked for one hour at 37°C.
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2D and 3D MRI time-lapse series reveal that pregastrular movements change the tissue architecture of the DMZ at earlier stages and in a different fashion than previously appreciated.
Several stacks were taken continuously to get time-lapse series of microglial action.
Digital images of live embryos were processed with Photoshop (Adobe Systems, USA) and time-lapse series were processed with ImageJ (NIH), adjusting background brightness and contrast.
The possibility to breed this line in homozygosis allows stronger intensity of the fluorescent signal and better conditions for the acquisition of long time-lapse series.
The aperture and exposure were set at 70-slit and 100 ms, respectively, and images were captured at 2 s intervals for total time of 7 min for a time-lapse series.
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