Remarkably, time-lapse ratio imaging at 5 s intervals showed that a flash of high FRET appeared in the bleb periphery just after protrusion, and was maintained until bleb retraction.
These methods were applied to automatically track and quantify the time-lapse FRET ratio of Raichu-RhoA biosensor at subcellular regions in live HeLa cells, and to analyze the polarity of intracellular regions with high RhoA activities.
The signs of the impacts, i.e., increase or decrease, depend on the static stability and total water mixing ratio lapse rates of the atmosphere.
Proportional hazards modelling will be used to analyse the median time to lapse by trial arm; hazard ratios (HRs) will be reported with corresponding 95% CIs.
Dynamics of nucleus/cell GFP-Nrf2 fluorescence intensity ratio obtained from time-lapse image sequences revealed that Nrf2 undergoes oscillatory cytoplasm-nucleus translocation.
Upon addition of the stabilizer drug, time-lapse microscopy revealed an increase in the GFP/mCherry ratio over time, which reflects the translation rate of the GFP.
The NC ratio of GFP-Smad2 was measured over time by time-lapse microscopy (n = 3 embryos).
(H ) Time-lapse images of a eGFP-centrin1/CENPA 2:1 cell as analyzed in G. Half-spindle ratio R and time before anaphase are indicated for each frame.
In total, 27 to 29 compartments of seven different aspect ratios (1.2, 1.5, 2.0, 2.5, 3.5, 4.5, 5.5) on the same time lapse movie were analyzed.
We estimated adherence by calculating a weekly drug possession ratio that incorporated an individual's pill count data and any lapses in delivery that could have occurred because of drug holidays or being away from home.
