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(A, B ) Corynebacterium glutamicum L-form strain grown in nutrient broth (NB /L-form supportiNB /L-form(Msupporting-cycloserine (A ), and Escherichia coli L-formediumin RMSM5 (ΔmurA) growithn nutrient agar/MSM (B ), were observed by time lapse phase contrast microscopy.
Normal elongated HeLa cells exhibit dynamic protrusive membrane activity at the ends of the longitudinal axis of the cell, and occasional activity at the cell sides, as assessed by time lapse phase contrast analysis of cells cultured on poly- l-lysine coverslips (supplementary material Movie 1).
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Colonies were cultured on Matrigel without feeder cells, treated with pertussis toxin or cholera toxin, and imaged by time-lapse phase contrast microscopy.
Using time-lapse phase contrast microscopy with an inverted microscope we have been able to successfully demonstrate the chemotaxis of MV3 melanoma cells towards a 10% Foetal Bovine Serum (FBS) gradient, with cell paths tracked using the ImageJ MTrackJ plugin (Fig. 4).
(E ) Quantitation of the percent of cells that migrate from time-lapse phase contrast videos.
(D ) Quantitation of the percent of cells that migrate from time-lapse phase contrast videos, treatments as in (C ).
(D ) Quantitation of the percent of cells that migrate from time-lapse phase contrast videos, treatments as in (B ).
(E ) Quantitation of the percent of cells that migrate from time-lapse phase contrast videos, treatments as in (C ).
Time-lapse phase contrast microscopy was performed using an automated IncuCyte phase contrast microscope (Essen Instruments, Ann Arbor, MI).
A time-lapse phase movie of the migration of cells was recorded every 3 min over a period of 2 hours.
The response of the breast cancer cell line MCF-7 was studied using time-lapse phase contrast microscopy and multi-parameter analysis for cell death using Array Scan.
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