Because these inevitable lapse periods can be incredibly vulnerable.
Nonetheless, out of approximately fifteen hundred total divisions observed, we were able to track 221 daughter pairs with 100% confidence to the end of time-lapse period.
In order to assess the fate of daughter cells from each division, we tested the possibility of tracking the daughter cells of all divisions, regardless of when they take place, to the end of entire time-lapse period.
The size and shape of SN oocytes during this time-lapse period remained mainly unchanged and, at the bright field, we did not observe specific marker features.
Migration velocity was recorded as an average over the full 24 hour time period, and as the average velocity with respect to each time point throughout the 24-hour time-lapse period.
Out of the 29 iACs we followed with somas situated one or more cell body diameters apical of the IPL at the end of the imaging session, all were seen at least once during the time-lapse period to be directly adjacent to the IPL, only to move away from the IPL later in the movie (Fig. 4A).
Full acquisition of YFP fluorescence measurements in a single cell over the entire time-lapse period (usually at least 18 h) proved difficult because of the high motility of MEL202 cells, which resulted in many cells leaving the field of view.
Exposure times and time-lapse periods for most experiments were set using image collection software (MetaMorph 7.5; Molecular Devices Inc., Downingtown, PA), which drives an external shutter (Uniblitz LS6; Vincent Associates, Rochester, NY).
And she was known to lapse into periods of "sorrowful rage," in which she would curse and keen.
