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To answer the questions, careful observation of LHI with time lapse analysis is needed.
Thus, cells were transfected with EGFP-p27T198E or -p27T198V and 1 day later plated on FN-coated dishes and followed for 2 hours using a fluorescence-based time lapse analysis.
Time lapse analysis revealed that the actin::GFP phenotype is dynamic with accumulations dissipating within dendrites, while simultaneously new accumulations can form within a different part of the same dendrite (Figure 2D F).
Our observations (Fig. 4, Movies S13 and S14) and previous time lapse analysis of vital dye or GAD67-GFP labeled neurons in the marginal zone indicate that marginal zone interneurons can migrate in multiple directions [22], [27].
(C ) Time lapse analysis of GFP-GPAT4 targeting to LDs.
(D ) SPC110 and spc110 T18D cells with SPC97-GFP were analyzed by time lapse analysis.
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The phenotypes found in this time-lapse analysis therefore confirm the histological and microarray analysis of the mutants.
These results were corroborated by video time-lapse analysis of migrating CR cells in explant cultures.
We prepared CH explants as above in Fluorodish cell culture dishes (World Precision Instruments, Sarasota, FL, USA) and 8 h later, we performed the time-lapse analysis.
Time-lapse analysis of >100 individually ablated axons for periods of up to a year reveals a surprising inability to regenerate even in a glial scar-free environment.
In conclusion, the available studies are too heterogeneous for firm conclusions to be drawn on the predictive value of time-lapse analysis for embryo aneuploidy screening.
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