Sentence examples for lane standard from inspiring English sources

Samples were processed using the 16-capillary 3130 × l Genetic Analyser (Applied Biosystems Inc .. 0.5 μl reaction products were mixed with 0.4 μl Internal Lane Standard 600 ROXTM size standard (Promega, WI, USA) or GeneScanTM 500 ROXTM size standard (Applied Biosystems, UK), in 9 μl Hi-Di Formamide (Applied Biosystems, UK).

These values for low speed situations are listed in Table 3. Open image in new window Fig. 2 The transverse remaining width and lane sharing Table 3 The critical value of transverse residual width W S Vehicle type of the parking lane Standard width(m) Travelling speed km/h) 10 20 30 40 Car 2.0 5.3 5.5 5.7 5.9 Truck 2.55 5.7 5.9 6.1 6.3.

A 1.0 µl aliquot of the amplified product was added to 9.5 µl of deionized formamide (Applied Biosystems) and 0.5 µl of ILS-600 internal lane standard (Promega).

ABI GENESCAN software was used to size fragments based on internal lane standard GeneScan 500 [Rox].

A mixture of 8.5 μl formamide (HiDi, Applied Biosystems) and 0.5 μl internal lane standard (Rox500, Applied Biosystems) was prepared and 1 μl of the PCR-product was added.

Samples were prepared for separation and analysis by adding 1 μl of 1 10 dilution amplified sample or allelic ladder to 10 μl of Hi-Di™ Formamide (Life Technologies™) and 1 μl of CC5 Internal Lane Standard 500 (ILS).

This problem can be reduced by including internal lane standards that allow normalization of individual lanes.

The fluorescent-labeled products were separated and detected by using a model 3730xl Automatic Sequencer (ABI); internal lane standards (Liz600; ABI) and the GeneScan program (ABI) were used to determine produce sizes.

The HEX-labeled fluorescent PCR products were mixed with an internal-lane standard (GS-500 TAMRA) and separated on a 6% polyacrylamide gel using a 96-well ABI 377 automatic sequenator.

PCR products were multiplexed and 1 μL of the diluted mix was added to 8.75 μL Hi-Di formamide and 0.25 μL of an internal lane size standard (Genescan™ 400HD-ROX Standard; Applied Biosystems) and run on an ABI 3130xl Genetic Analyzer (Applied Biosystems), following the manufacturer's protocol.

Lanes M protein molecular mass standard; lane K EngK; lane S ExgS; lane H EngH Fig. 3 Specific activities of recombinant EngK and ExgS against acid-swollen cellulose.