A green bike lane separates the domesticated zones from the curb, like a moat patrolled by speeding bicycle messengers.
A single country lane separates a long row of beachfront rental cottages from salt marshes on the leeward side.
Suddenly, several lanes of cars and buses are zipping past, but Calvo pays no heed – we are on a smooth, green-tarmacked bike lane, separated from motor traffic by both a raised kerb and a waist-high fence.
Those of us on two wheels are funnelled onto a brand new, billiard table-smooth bike lane, separated from the metal behemoths by a raised kerb, cosseted with our own mini traffic lights.
There's nothing quite like riding a bike in the comfort of your own lane, separated from speeding traffic.
Immunoblotting was performed on cell lysates and medium proteins (20 mg protein per lane) separated by SDS-PAGE before transfer to nitrocellulose membrane (Amersham Bioscience, Uppsala, Sweden).
Extracts were normalized for total protein (10 µg per lane), separated using 4% 20% linear gradient SDS-PAGE (Bio Rad, Hercules, CA) and electroblotted.
One nanogram of purified SOLD1 recombinant protein was loaded on each lane, separated by SDS-PAGE, and electrophoretically transferred onto a polyvinylidene-difluoride membrane.
Equal amount of lysates were loaded (20 µg of total protein per lane), separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane.
Lysates were normalized for total protein (10 µg per lane), separated using 4% 20% linear gradient SDS-PAGE (Bio Rad, Hercules, CA), and electroblotted.
