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The landmarks were recorded on standardized digital photographs with the aid of TPS Dig [ 47].
First, the locations of 12 anatomical landmarks were recorded from the head and jaw of each specimen.
Coordinates of 8 landmarks were recorded for each LPJ using tpsDig 2.11 ([ 92], for landmark positions see Figure 1).
Landmarks on the images were picked along cell lineage boundaries, and coordinates of edge landmarks were recorded.
The landmarks were recorded by one individual using MeshLab (V 1.3.0) [ 27], and the corresponding x,y,z coordinate locations were used for subsequent shape analysis.
For this approach, the locations of nine anatomical landmarks were recorded from the foot of each specimen (points 1-9: Figure 1B).
Similar(51)
The day of appearance of developmental landmarks was recorded and used as the unit of analysis.
For Procrustes ANOVAs of complex symmetries in the framework of matching symmetry, the units of analysis are the repeated parts (e.g. petals of flowers) on which landmarks are recorded.
Semi-landmarks were recorded as equidistant points along two (for M, M2 and M) or four (for M3) curves that were delimited by extra landmarks, later one transformed as sliding semi-landmarks (SI-Figure 1).
The order that each landmark was recorded is also presented (see additional movies material).
The designation A (anterior) or P (posterior) after the landmark name indicates in which position(s) the landmark was recorded.
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