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We performed a true 3D analysis because the location of each landmark was analyzed relative to the others.
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Digitized landmarks were analyzed for components of shape variation in a thin-plate spline analysis using tpsRelW (Rohlf 2008).
The subset of 20 μg/g SAG-treated euploid mice that were distinct in the previous analysis of all cranial landmarks, are also distinct when the facial landmarks are analyzed separately (Fig. 4A).
In addition to analysis of the overall cranial shape using 41 cranial landmarks (supplementary material Fig. S1a; Table S1), subsets of landmarks representing the facial skeleton (24 landmarks), cranial vault (7 landmarks) and basicranium (10 landmarks) were analyzed separately (supplementary material Table S1).
The assessment of intra-observer variability and reproducibility of landmark location and measurement errors was analyzed by retracing the 10% randomly selected cephalograms after a gap of 15 days.
Body shape variation was analyzed with landmark-based morphometric techniques and multivariate statistical methods [ 35].
Global methylation was analyzed using restriction landmark genomic scanning (RLGS) as previously described [ 17].
The shape of each module was analyzed using a principal component analysis on the Cartesian coordinates of the landmarks and sliding landmarks after a Procrustes superimposition.
Her wardrobe was analyzed and re-analyzed.
The landmark coordinates were analyzed using principal component analysis (PCA) (Miriam 2004) and canonical variate analysis (CVA) to identify shape trends in the various subgroups (Bilfeld et al. 2013).
The landmark coordinates were analyzed using principal component analysis (PCA) (M Z 2004) and canonical variate analysis (CVA) to identify shape trends in the various subgroups (Bilfeld et al. 2013).
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