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To develop loop-mediated isothermal amplification (LAMP) to detect Nosema ceranae (N. ceranae) in honeybee samples.
Our current study demonstrated that the application of LAMP to detect Zika virus was superior to traditional methods with a high sensitivity and specificity.
Cells were observed to detect ROS presence in cytoplasm using an inverted microscope (Nikon Eclipse TS 100) under minimum illumination conditions with a regular lamp to detect cells and then, using different filters (488 nm excitation and 530 nm emission for carboxy-DCF) to visualize fluorescence.
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The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated.
In this study, we report the development of PfSNP-LAMP to detect nucleotide polymorphism in the dhfr gene associated with N51I mutation and antifolate resistance.
The ability of RT-LAMP to detect bovine rotavirus was further evaluated with 88 bovine rectal swab samples.
A primer set was designed for RT-LAMP to detect enterovirus preferably those with PV-like 5'NTRs of the viral genome.
In the present study, we evaluated the ability of H7-RT-LAMP to detect a novel influenza A (H7N9) virus that was identified in humans and birds in China and developed a novel one-step RT-LAMP assay that was specific for the neuraminidase (NA) gene of the H7N9 influenza virus.
We have developed a loop-mediated isothermal amplification (LAMP) assay to detect the presence of the transposase gene insertion element IS1111a of C. burnetii.
The objective of this study was to develop and evaluate a loop-mediated isothermal amplification (LAMP) method to detect infectious laryngotracheitis virus (ILTV) from commercial broiler and layer flocks in southern China.
Guan et al. [ 15] found the optimization of different primer concentrations and the ratios between inner primers (FIP and BIP) and outer primers (F3 and B3) to be essential for the development of a LAMP assay to detect genetically modified soybean events.
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