The health of the cornea in eyes of live rabbits was examined by visual clinical and slit-lamp microscopic (BX 900 Slit Lamp, Haag-Streit-USA, Mason OH) examinations before and after AAV5 application in normal and diseased (hazy or neovascularized) rabbit corneas by two ophthalmologists and a researcher, independently and in a blinded manner while animals were under general anesthesia.
Keratic precipitates existed in 2 of 35 eyes by slit-lamp microscopic examination.
Because rabbits have large eyes amenable to slit-lamp microscopic examinations, and considering their gentle nature and relatively low cost to maintain, rabbit models are well-suited to study the development of DE.
Using an established, semi-quantitative scoring system, FITC staining of the corneal/conjunctival surface was evaluated by slit-lamp microscopic eye examination of each eye (n = 10), and the corneal and conjunctival areas were scored on a scale of 1 to 4 (shown in Additional File 6) by an observer unaware of the type of treatment given to each mouse.
The author performed slit lamp biomicroscopy, microscopic analysis of lens sections, and drafted the manuscript and subsequent revisions The pre-publication history for this paper can be accessed here:.
This microscope was equipped with a 12 V, 100 W halogen (Xe/Hg) lamp and a color filter for microscopic imaging.
b I-V curves of the Er-CdS NR device under illumination with an incandescent lamp; the inset shows the optical microscopic image of a single Er-CdS NR detector.
The ALP activity was observed in brightfield microscopic, using a high lamp voltage in order to make the ALP+ cells visible through the thickness of the PCL film.
Lamp voltage was set to 6 V so that the microscopic visual assessment using the oculars can be done without changing settings other than the light path.
Microscopic analysis was performed using an Axioplan II imaging microscope (Carl Zeiss) with an HBO-103 mercury lamp and filter sets for FITC, Cy3.5, Texas Red, Cy5, Aqua, and DAPI.
Microscopic examination was performed using a Zeiss Axioimager M1 epifluorescence microscope equipped with a 120-W HBO lamp.
