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When turbidity was used as a detection method, LAMP could be conducted with samples spiked with P. falciparum DNA without requiring an external power source, maintaining the same limit of detection as LAMP conducted with a conventional laboratory heat source.
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Laboratory tests on various LED lamps are conducted and the electrical characteristics of LED lamps are tapped under different conditions.
LAMP can be conducted in 1 h and requires only a simple heating device for incubation.
Three experiments, each comparing a variation of the simulated udder and/or a heat lamp only were conducted and the ratio of piglets from each litter using a modified simulated udder to all live piglets in the litter was determined.
Among 8447 clinical isolates isolated at 36 medical institutions, the LAMP method was conducted for 191 strains that were resistant to aminoglycosides based on the results of antimicrobial susceptibility tests.
LAMP can be conducted under isothermal conditions and does not need expensive thermocyclers [ 120].
Slit lamp examinations were conducted at each visit and the findings were graded on a scale from 0 to 3 (0: normal; 1: mild; 2: moderate; 3: severe).
Fundoscopic examination in mydriasis using slit lamp biomicroscopy was conducted by the same ophthalmologist who was unaware of the clinical status of the patient.
In addition, to evaluate safety, slit lamp biomicroscopy was conducted to assess pathological changes, tonometry was conducted to determine IOP, blood pressure and heart rate were measured, concomitant therapy drugs were noted, and complaints and adverse events (AEs) were recorded.
DNA from a N. gonorrhoeae standard strain, as well as from 26 genital secretion samples of gonorrhea patients, were isolated and used for loop-mediated isothermal amplification (LAMP) assay, which was conducted using either an automatic real-time PCR analyzer or a water bath.
Taking into account the advantages of LAMP, direct detection of MDV DNA in poultry dust has been conducted in this research.
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