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About 60.4% (96/159) of cattle samples were positive by LAMP, compared to 30.0% (46/154) of water buffalo samples that were positive.
However, in contrast to what was reported by Poon et al. the sensitivity and specificity of LAMP compared to a nested PCR based on primers designed by Singh et al. [18] were shown to be 79.1% and 58.3% respectively when heat treatment for DNA extraction was used [15].
The higher sensitivity of LAMP compared to parasitological methods and PCR is also of significance in regard to the search for new diagnostic tests for sleeping sickness.
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RT-LAMP was compared to real-time RT-PCR with SYBR Green I and melting curve analysis, using serial dilutions of total RNA extracts.
The findings of RT-LAMP were compared to those obtained by qRT-PCR.
Echovirus 11 (belonging to HEV-B) and enterovirus 71 (EV71) strains (belonging to HEV-A) showed lower sensitivity in the RT-LAMP reaction compared to PV(Sabin) strains.
The sensitivity of the LAMP was 100% compared to egg positive samples.
The RT-LAMP efficiency was compared to virus isolation and a commercially available enzyme immunoassay (EIA) for RSV detection (BD Directigen EZ RSV test™), using nasopharyngeal aspirates from 59 children with respiratory tract infections.
Silencing of USP22 and PI4KCA did not affect co-localization with LAMP-2 when compared to untreated cells (Fig. 5A and C), indicating successful modulation of phagosome biogenesis.
Cells depleted for KIF5B and exposed to 125 μF did not display any significant change in surface LAMP-1 as compared to control treated cells exposed to 125 μF (Fig. 3D).
The diagnostic performance of RT-LAMP assay as compared to qRT-PCR was calculated using web-based CEBM Statistics Calculator (http://ktclearinghouse.ca/cebm/toolbox/statscalc).ca/cebm/toolbox/statscalc
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