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PMA (Biotium, Hayward, CA, USA) was added to the samples at a final concentration of 50 μM and the samples shaken in the dark for 5 min were placed in the PMA lamp apparatus (LED-Active Blue, Ingenia Biosystems, Barcelona, Spain) for 15 min.
The base for your lamp should be sturdy enough to stand on its own, even after you add lamp apparatus and a shade on top.
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A lamp illuminated the apparatus and a video camera was mounted 50 cm above the apparatus to record trials for analysis.
A lamp illuminated the apparatus, and a white shelf, 50 cm from the top of the apparatus, created a ceiling on which a video camera was mounted to record trials.
The only problem now is that he has to hold the dish juuust right in order to keep connected, a problem he says he might attempt to solve by attaching the apparatus to an adjustable lamp arm.
These were performed using a Hi-Tech stopped-flow apparatus with a xenon mercury lamp (TgK Scientific).
All stopped-flow fluorescence measurements in this investigation were performed at 30 °C using a Hi-Tech stopped-flow apparatus with a xenon−mercury lamp (TgK Scientific, U.K .. Solution conditions and wavelengths for individual measurements are given in the figure legends.
The degradation chamber apparatus had a filtered xenon lamp (cut off > 290 nm) and an irradiation of 0.3 W/m2/nm @ 340 nm.
Therefore, UV-light using a collimated beam apparatus with a low pressure lamp which emitted monochromatic radiation with a peak at 254nm was used to assess the suitability and sensitivity of both detection assays for quantifying DNA damage.
Stopped flow experiments were carried out using a HiTech SF-61DX2 apparatus (TgK Scientific) with a xenon mercury lamp and operated by Kinetic Studio software (TgK Scientific).
The optimized UV-LED collimated beam apparatus and a low-pressure mercury lamp collimated beam apparatus were used to determine the UV fluence-response of three surrogate microorganisms (Escherichia coli, MS-2, T7) to 255 nm UV-LEDs, 275 nm UV-LEDs, and 254 nm low-pressure mercury lamps.
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