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The animals were housed under sterile conditions in a laminar flow environment with ad libitum access to food and water.
Whilst receiving the study intervention, if the patient's clinical team decided the patient required additional vitamins, minerals, or micronutrients (see Table S2), they were delivered via a separate infusion bag and not admixed with the study parenteral nutrition; as such, requirements for preparation of the study parenteral nutrition in a laminar flow environment were avoided.
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After a defined rotational speed was reached, the cells were cultured under Earth gravity, in a near laminar fluid flow environment (i.e., a free-fall state).
The skin was thoroughly washed successively with tap and distilled water, drained and totally covered with 70% ethanol which was allowed to evaporate on its own in a laminar flow hood sterile environment.
All working steps were carried out under the laminar flow to ensure a sterile environment.
Frictional coefficient ratios (FCRs) for both laminar and turbulent flow environments are computed as a function of the through Reynolds number and the rotational Reynolds number.
All PCR steps were conducted in a sterile environment, under a laminar flow hood, and all surfaces, tubes, and equipment were UV irradiated between each PCR.
Twenty-four nude mice (6 to 8-week-old females) of the NMRI strain obtained from Harlan Winkelmann GmbH (Borchen, Germany) were maintained in groups of six animals per cage in laminar flow hoods in a pathogen-free environment.
The mixture of N-free Hoagland's solution and Phytablend was autoclaved for sterilization, and media was poured into sterile tubes within a laminar flow hood to maintain a sterile environment.
This printer can be used in a semi-clean environment such as a laminar flow bench.
Animals were maintained in laminar flow cages in a specific pathogen-free environment at the University of Pittsburgh Pittsburghh, PA, USA) animal facilities and given standard diet and water.
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