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Nerves from 10-month-old Cx3cr1GFP/+ DBA/2J mice including the retro-orbital, myelinated segment (1-1.5 mm post-lamina), were prepared as 1-µm-thick plastic cross-sections, and stained with a modified paraphenylenediamine (PPD) protocol and Toluidine Blue (Anderson et al., 2005; Calkins et al., 2005; Inman et al., 2006; Sappington et al., 2003).
Cuticles were prepared from the leaf lamina region of the counterpart of the first specimen (figure 3).
Samples were prepared from each set of laminae, both for microanalysis and for mechanical testing, under proposed configurations.
Cell suspensions were prepared from spleen, MLNs, and colon lamina propria by methods modified from previous report.
One-dimensional (1-D) and two-dimensional (2-D) mesophase pitch-based graphite fiber (MPGF /acrylonitrile butadiene styrene (ABS) resin composites were prepared from unidirectionally arranged MPGF/ABS resin laminae by a hot-press method.
were prepared.
Positive cells superficial to the basal lamina were not counted.
The boundaries of the internal lamina were drawn on the basis of the MD image that shows the following MD values: WM ∼0.3 × 10−3 mm²/s, deep lamina ∼0.8 × 10−3 mm²/s, internal lamina ∼0.6 × 10−3 mm²/s, superficial lamina ∼1 × 10−3 mm²/s.
Lamina propria mononuclear cells (LPMCs) were prepared as described above.
The nuclear envelope lamina is reversibly depolymerized during mitosis.
The yellow structures on the lamina are spores.
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