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The number of human lamin A/C+ nuclei, human spectrin+ fibres, human spectrin+ fibres containing human lamin A/C+ nuclei and the number of human lamin A/C+ nuclei inside or outside basal lamina were counted using MetaMorph software and compared using either Student's t test or one-way ANOVA.
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In addition, a significant proportion of double-labeled neurons were counted in lamina I, representing 15±4% (n = 3 rats) of retrogradely labeled neurons in lamina I (Figure 5C).
In this sense, only nuclei between the basal lamina and the plasmatic membrane were counted.
To calculate the percentage of CD68, IgA and CD3, one thousand lamina propria cells per biopsy were counted.
At 5 weeks post injury/transplantation, NeuN+ neuron cell bodies were counted in laminae 4 to 9 on the injured, right hand side of the spinal cord (Tables 1 and 2).
In the present study, c-Fos-immunoreactive cells were counted as combined laminae I-II, III-IV, V-VI, and VII-VIII, respectively.
Five nonadjacent sections from the L4-L6 lumbar spinal cord were randomly selected, and the numbers of immunoreactive (IR) cells in the superficial lamina (lamina I-II) of the dorsal horn in each section were counted by an observer blind to the treatment.
In each case, the numbers of neurons were counted in Rexed's laminae VIII and IX of the lumbar spinal cord.
The number of nuclei in the center and the basal lamina for each muscle fiber of bicep muscles were counted.
Points overlying staining of interest were counted along with intersections of test lines with basal lamina.
Neurons in lamina 9 innervating the external urethral sphincter (ExU9) and the external anal sphincter (ExA9) were counted in sections at the level of L6.
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