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Changes in lamina structure are also induced by mutations in lamin A or lamin B genes.
Based on the distribution of curvature and intensity values in the cell populations, we suggest that when cells senesce changes in lamina structure are predominantly associated with a change in intensity, whereas in cells undergoing apoptosis curvature values are also increased.
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In our 3D cell culture system neither changes in epithelial cytokeratin expression nor the disappearance of basal lamina structures were detected during the culture period, indicating that the glandular cells were fully differentiated.
In addition to decreases in the overall density of LMNA and LMNB in LmnaDhe/+ fibroblasts, perturbations to nuclear lamina structure also were detected.
The program for identification of the nuclear lamina and quantification of the structure is completely described in [ 10].
They also have the advantage that the direction of the filaments in a multi-lamina structure may be aligned with the direction of the major loads at a particular point, resulting in a more efficient design.
Quantification of the nuclear lamina structure could, therefore, be an objective tool to describe changes in the lamina structure and to identify mutant or unhealthy cells within a cell population.
In this way, the manchette-like perinuclear structure is connected to the nuclear lamina, thus enabling the motility of perinuclear microtubules and motor proteins to influence the activity of the nuclear lamina.
Weakening of the nuclear lamina further enhanced envelope remodeling, suggesting that envelope structure is capable of counterbalancing chromatin compaction forces.
8, 25 Such a structure is usually also called a 'dental lamina' in papers.
MTs nucleated at the centrosome are fragmented and kinetochore structure was significantly altered showing only two laminae with few MTs inserted into this structure.
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