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The parameters evaluated were luminal diameter, external elastic lamina diameter (vessel diameter), maximal neointimal thickness, external elastic lamina area (vessel area), luminal area, neointimal area, and area stenosis, which was determined as area stenosis = 100 – ([lumen area · 100]/vessel area).
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The 8 mm diameter transparent lamina has microperforations to allow permeability facilitating drainage of exudates at the surgical site (Fig. 1).
Plant height, basal stem diameter and lamina length of all leaves were determined in the same manner as for the focal plants.
Due to the role of NG2 in pericyte biology, pericyte ensheathment of endothelial cells is diminished during the early stages of tumor vascularization in NG2 null mice, accompanied by reduced pericyte maturation, reduced sprouting of endothelial cells, diminished assembly of the vascular basal lamina, and smaller vessel diameter.
By three days post-injection, the number of labeled large diameter cells in lamina IX increased and labeled neurons appeared in laminae IV VII.
Image analysis of isolectin B4-positive fibers within the lamina propria revealed a mean diameter of 1.089±0.095 µm with a range of 0.728 1.361 µm (n = 7), consistent with the morphology of bladder C fiber nociceptor axons in dorsal root ganglia [24].
In the absence of NG2, pericyte ensheathment of endothelial cells was reduced, along with reduced pericyte maturation, reduced sprouting of endothelial cells, reduced assembly of the vascular basal lamina, and reduced tumor vessel diameter.
As the thickness is very small, the volume gradient in the same direction should be tuned with changing of the wire diameters in each lamina.
The internal and external diameter were calculated according to the lamina elastic internal and the lamina elastic external indicated by EVG staining in a blinded manner by a single observer using Image Pro Plus 6.0 software (Media Cybernetics) [ 31].
On the second lumbar vertebra, the spinous process was removed and an ∼1-mm-diameter hole was drilled on the left side of the lamina in which a stainless-steel screw (1 mm diameter; 3.7 mm long) was inserted to anchor the implant.
Upon cooling to room temperature, the scaffold, its adherent cells, and the remaining cell sheet detached and were collected on a mandrel to generating tubular constructs with circumferentially aligned smooth muscle cells which possess contractile gene expression and a single layer of electrospun scaffold as an analogue to a small diameter blood vessel's internal elastic lamina (IEL).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com