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Morphological changes of the L2 dendrites were examined by tracing the outline of dendrites and axon of L2 cell cross sections at the same lamina depth, 15 µm from each L2 cell soma (Fig. 1C inset).
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To determine whether: (1) change in lamina cribrosa depth occurs more frequently than change in neuroretinal parameters in glaucoma, and (2) Bruch's membrane or anterior sclera should be used as a reference plane when measuring laminar depth.
To determine whether lamina cribrosa (LC) depth (LCD) and LC thickness (LCT) are associated with a faster rate of progressive retinal nerve fiber layer (RNFL) thinning in primary open-angle glaucoma (POAG).
The blinded assessors were asked to measure the angle of the thyroid cartilage using lines that joined the apex of the cartilage to the estimated junction of the thyroid lamina at a depth of 1 cm from the apex.
Following this surface estimation, the mean anterior lamina cribrosa surface depth (ALCSD) was calculated as the mean perpendicular distance from a plane best-fit to the BMO point cloud to the thin plate spline surface (fit to the marked ALCS points) for locations within the BMO ellipse [ Fig. 2(b)] [ 8].
These findings indicate that lamina-specific visual connections are generated through the selective stabilization of correctly targeted axon arbors and suggest that the decision to maintain or eliminate an axonal projection reflects the molecular compatibility of presynaptic and postsynaptic neurons at a given laminar depth.
A branching blood vessel can also be distinguished in the lamina propria at the depth of ~600-700 μm.
Finally, the experiment of scanning a lamina by the large depth-of-view portable 3D laser scanner used by an industrial robot IRB 4400 is also employed to demonstrate the effectiveness and high measurement accuracy of our scanning system.
Observation of the images indicated variations in the shape and arrangement of the lamina pores at different depths.
With tracking, we were able to visualize the porous structure of lamina cribrosa at larger depths, approximately 430 µm below the bottom of the ONH cup (indicated by a white dashed line in Fig. 10).
L2-cells form uniform arrangements of short, radially-directed dendritic branches throughout the depth of the lamina neuropil.
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