The myofiber basal lamina contains a network of ECM components that include collagen IV, laminin, perlecan, nidogen, collagen VI, fibronectin and other proteoglycans and glycoproteins [179,180].
In comparison with the C57BL mouse, representative images from SBEM volumes from the glaucomatous glial lamina contained a higher number of dense deposits in degenerative axons as well as hypertrophic astroglial activation.
If we compare these layers with the segmentation employed in this study, we find a rough correspondence of the cytoarchitectonic layer I to the superficial lamina containing Exner's stripe; layers II and III to the external lamina; layer IV, which also contains the Stria of Gennari, to the internal lamina; and layers V and VI to the deep lamina.
The spiral lamina containing the SG was carefully separated from the modiolus and immediately transferred into primary cell culture medium.
Each pellet was resuspended in high salt buffer (2 ml/100 g fresh weight lamina), containing 100 mM KCl, 15 mM HEPES.KOH pH 7.6, 5 mM MgCl2, 1 mM EDTA pH 8.0, 1 mM DTT, 5 μg/ml leupeptin and lysed by incubating on ice for 45 min with vigorous shaking.
Accordingly, the cellular region that overlaps the border between the inner part of lamina II and lamina III contains a substantial number of local dorsal horn interneurons, which themselves may activate NS neurons [25], most likely through polysynaptic connections [30].
The inclined UD lamina was established, containing a braided yarn with the same diameter and fibre volume fraction as in the 3D braided composite at the actual microstructure.
The number of distinct fiber orientation angles and the number of plies each lamina contains were predetermined.
The lamina contains five types of lamina monopolar neurons (LI-L5).
Basal lamina contains several extracellular matrix (ECM) proteins such as collagen, laminin, fibronectin and proteoglycans.
