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(C ) Left Panel: Treatment in the presence or absence of MLN4924 for 3 hr reveals that the inhibitor treated explant has lagging expression of cLfng compared to the control contralateral explant.
(D – F ): Treatment of chick PSM explants in the presence or absence of Roscovitine (D ), DRB (E ) or XAV939 (F ) for 3 hr reveals that '+' explants have lagging expression of cLfng, with one less somite formed than the '−' explants.
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Note that the HMM also assigns high scores to time courses with similar but lagged expression patterns.
Again, the improvement in cardiac function paralleled the reduction in transgene expression with functional changes lagging transgene expression changes by about 24 hours as determined by QRT-PCR (Figure 2).
If the LEA gene we have identified is indeed involved in dehydration resistance or cold hardiness, the lagging LEA expression we observed in the evg mutant is consistent with the impaired cold hardiness response previously observed in seasonal LEA expression in evg and deciduous genotypes of peach [ 47].
As with omi, hax-1 showed a similar, early increase in expression although this lagged omi expression by at least one hour (figure 4A).
Thus, the expression of CYP8B1 also increases over time, although it lags the expression of its regulatory receptors Hnf4a and Nr5a2.
Time lagged co-expression relationships are those in which the expression patterns of two genes are better correlated when a time shift is introduced [ 32].
GFP expression follows the wave of somitogenesis, with the posterior-most somites lagging in GFP expression.
5430 (65.5%) of the 8284 edges were characterized by time lagged co-expression relationships.
Under EB differentiation conditions, eGFP fluorescence peaked during days 3 5, also lagging behind endogenous T/brachyury expression by about a day (not shown), diminishing thereafter as expected (day 4 shown in Figure 5F).
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