These sections were stained by thionin (Sigma) and 4',6'-diamidino-2-phenylindole (DAPI, Sigma) to investigate the persistence of DNA within osteocyte lacunae [9].
Additionally at postlarval stages we see evidence of active bone resorption, both using vital labeling (Figure 4B, *), and by visualizing matrix depressions with the appearances of Howship's lacunae [30].
While metabolic factors such as diabetes can influence the spacing of the lacunae, the overall structure of the network is robustly determined within a very narrow range of the number density of canaliculi branching out of individual lacunae (~1 canaliculi per 5 μm lacunar surface in adult mice).
Despite exhibiting mineralisation of soft tissues and osteocyte lacunae, Enpp1 −/− mice display a decreased trabecular and cortical bone volume [20].
16 Ultrasound features such as placental lacunae, 15 and MRI features such as uterine bulging, 17 have been documented as being suggestive of placenta accreta, increta, and percreta.
By discriminating 'cartilage' from the 'lacunae' using the ImageJ data, we could show that administration of IOP resulted in a 10.92% decrease of lacunae (p=4.27×10−5) on day 35 of treatment.
Both streptococci and Haemophilus species are potent biofilm-forming pathogens that may result in chronic persistence in the adenoid lacunae [ 21, 23, 26].
11, 12 The severity of the lacunar state was rated from grade 0 to 3 as follows: 0, zero lacuna; 1, one to two lacunes; 2, three to four lacunes; and 3, more than five lacunes.
ON lesion was histopathologically characterized with trabecular bone containing considerable empty lacunae (Figure 3A, 3B and 3C).
Recently a fluorescence-based technique, "fluorescence after photo-bleaching," was employed to evaluate molecular transport in a lacuna [ 14, 27, 28].
The primary osteoclast driver is RANKL [ 78], although co-stimulators such as the cytokines IL-1β, IL-6 and/or TNF-α co-stimulate osteoclasts to secrete cathepsin K into the resorption lacunae [ 79, 80], resulting in degradation of the organic matrix of bone.
